Fig. S9
Kdm6b.1 MO2 inhibits regeneration. (a and b). Embryos were injected at the one-cell stage with approximately 1 nL control or Kdm6b.1 MO2 morpholinos (250 μM needle concentration). After 48 h, animals were sedated and caudal fin primordial amputated distal to the notochord. Animals were harvested 48 additional h later and fixed and photographed or stained with hematoxylin or PCNA antibodies as indicated (b). (c) Regeneration is abrogated up to 96 hpa in Km6b.1 knockdown animals compared to control animals. |