Fig. 5

Zebrafish embryos develop asymmetric lymphatic collecting ducts. A,B: Dorsal views, lyve1 expression within lymphatic trunks of 48 and 72 hours postfertilization (hpf) embryos. C,D: Dorsolateral views, lyve1 expression within lymphatic trunks of 80 hpf embryos. Expression of lyve1 within the thoracic duct (TD) of an 80 hpf larvae visible from the right side of the larva (arrowheads), but discontinuous from the jugular lymphatics to the TD on the left side (asterisk). E-H: Coronal (E,F) and transverse (G,H) sections confirmed the laterality of the collecting ducts (relative positions marked in top middle panel). I,J: Depletion of a bone morphogenetic protein (BMP) receptor, Bmpr2a resulted in reversal of the LR architecture of lymphatic ducts in 54 hpf embryos. Dorsal views of 54 hpf embryos (I); graph of morphant phenotype distribution (J). Blue arrows and bars denote normal lymphatic duct asymmetry; Black arrows and bars denote reversed lymphatic duct (LD) asymmetry.