Fig. S1
Zebrafish Foxd3 induces mesoderm in Xenopus animal explants. At the one-cell stage, Xenopus embryos were injected in the animal pole with 100 pg of Xenopus foxd3 (xFoxD3, lane 2) or zebrafish foxd3 (zFoxD3, lane 3), explants were prepared at the late blastula stage (stage 9) and analyzed by RT-PCR at the tailbud stage (stage 25) for the expression of Muscle Actin (somitic muscle) and Collagen II (notochord). EF1α is a control for RNA recovery and loading, intact embryos (Embryo, lane 4) served as a positive control and an identical reaction without reverse transcriptase controlled for PCR contamination (Embryo-RT, lane 5). |
Reprinted from Developmental Biology, 342(1), Chang, L.L., and Kessler, D.S., Foxd3 is an Essential Nodal-Dependent Regulator of Zebrafish Dorsal Mesoderm Development, 39-50, Copyright (2010) with permission from Elsevier. Full text @ Dev. Biol.