Fig. S4
- ID
- ZDB-FIG-100603-37
- Publication
- Piloto et al., 2010 - Ovo1 links Wnt signaling with N-cadherin localization during neural crest migration
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Identification of pacpar2.10 (ncadb>+/b>/b>-) mutants. (A) Schematic of the different ncad mutant isoforms. The wild-type product is 266 bp; mutants contain either a smaller 143 bp form or larger isoforms with as many as 50 additional nucleotides. (B,C) RT-PCR for ncad in pacpar2.10 heterozygous embryos injected with Ovo1 MO (B, all lanes; C, lanes 6-8) or with Rab11fip2 RNA (C, lanes 3-5). All embryos with ectopic dorsal NC cells (B, lanes 7-11; C, lanes 3,4,6,7) are heterozygous for pacpar2.10, whereas embryos whose NC cells migrated normally do not exhibit alternative spliced forms of ncad (B, lanes 2-6; C, lanes 5,8). Uninjected pacpar2.10 heterozygous control (C, lane 2). |