ZFIN ID: ZDB-FIG-100429-70
Wada et al., 2010 - Dermal morphogenesis controls lateral line patterning during postembryonic development of teleost fish. Developmental Biology   340(2):583-594 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Long-pec to Day 6
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Long-pec to Day 6

Fig. 6 Morphogenesis of the opercle correlates with neuromast patterning. (A–D) Time-course observations of control (A, C) and edn1-MO injected (B, D) embryos doubly transgenic for atoh1a:dtomato and cldnb:gfp (A, B), or HG21C (C, D). In morphant embryos, OP2 forms earlier than in control embryos (A, B). In control embryos, both OP1 and OP2 are located ventral to the opercle midline (C). In contrast, in morphant embryos, OP1 is located on the dorsal half of the opercle and moves away from OP2, which is located on the ventral opercle (D). Arrowheads and arrows indicate OP1 and OP2, respectively. (E, F) Single peridermal cell was labeled with rhodomine-dextrane and traced its fate, showing proliferation of peridermal cells relative to the neuromasts. (G) Schematic drawings of the developing opercular lateral line system in control and morphant embryo. The stage of the embryo is indicated in each panel. Scale bars: 50 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
EGFP nns8Tg; zf106Tg control Long-pec opercle anterior lateral line primordium IFL
Pec-fin opercle anterior lateral line primordium IFL
Day 4 opercular lateral line neuromast IFL
Day 5 opercular lateral line neuromast IFL
nns8Tg; zf106Tg + MO1-edn1 standard conditions Long-pec opercle anterior lateral line primordium IFL
Pec-fin opercle neuromast IFL
Day 4 opercular lateral line neuromast IFL
Day 5 opercular lateral line neuromast IFL
tcf7nkhg21cEt; nns8Tg control Protruding-mouth opercular lateral line neuromast IFL
Day 4 opercular lateral line neuromast IFL
Day 5 opercular lateral line neuromast IFL
Day 6 opercular lateral line neuromast IFL
tcf7nkhg21cEt; nns8Tg + MO1-edn1 standard conditions Protruding-mouth opercular lateral line neuromast IFL
Day 4 opercular lateral line neuromast IFL
Day 5 opercular lateral line neuromast IFL
Day 6 opercular lateral line neuromast IFL
Tomato nns8Tg; zf106Tg control Long-pec Not Detected opercle neuromast IFL
Pec-fin Not Detected opercle neuromast IFL
Day 4 opercular lateral line neuromast hair cell IFL
Day 5 opercular lateral line neuromast hair cell IFL
nns8Tg; zf106Tg + MO1-edn1 standard conditions Long-pec Not Detected opercle neuromast IFL
Pec-fin Not Detected opercle neuromast IFL
Day 4 opercular lateral line neuromast hair cell IFL
Day 5 opercular lateral line neuromast hair cell IFL
tcf7nkhg21cEt; nns8Tg control Protruding-mouth opercular lateral line neuromast hair cell IFL
Day 4 opercular lateral line neuromast hair cell IFL
Day 5 opercular lateral line neuromast hair cell IFL
Day 6 opercular lateral line neuromast hair cell IFL
tcf7nkhg21cEt; nns8Tg + MO1-edn1 standard conditions Protruding-mouth opercular lateral line neuromast hair cell IFL
Day 4 opercular lateral line neuromast hair cell IFL
Day 5 opercular lateral line neuromast hair cell IFL
Day 6 opercular lateral line neuromast hair cell IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
cntnap2ankhgn39dEt; nns8Tg + MO1-edn1 standard conditions Long-pec opercular lateral line neuromast premature, abnormal
Pec-fin opercular lateral line neuromast premature, abnormal
Day 4 opercle increased width, abnormal
Day 4 opercle shape, abnormal
Day 4 opercular lateral line neuromast mislocalised dorsally, abnormal
Day 4 opercular lateral line neuromast premature, abnormal
Day 5 opercle increased width, abnormal
Day 5 opercle shape, abnormal
Day 5 opercular lateral line neuromast mislocalised dorsally, abnormal
Day 5 opercular lateral line neuromast premature, abnormal
tcf7nkhg21cEt; nns8Tg + MO1-edn1 standard conditions Protruding-mouth opercular lateral line neuromast premature, abnormal
Day 4 opercle increased width, abnormal
Day 4 opercle shape, abnormal
Day 4 opercular lateral line neuromast mislocalised dorsally, abnormal
Day 4 opercular lateral line neuromast premature, abnormal
Day 5 opercle increased width, abnormal
Day 5 opercle shape, abnormal
Day 5 opercular lateral line neuromast mislocalised dorsally, abnormal
Day 5 opercular lateral line neuromast premature, abnormal
Day 6 opercle increased width, abnormal
Day 6 opercle shape, abnormal
Day 6 opercular lateral line neuromast mislocalised dorsally, abnormal
Day 6 opercular lateral line neuromast premature, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 340(2), Wada, H., Ghysen, A., Satou, C., Higashijima, S.I., Kawakami, K., Hamaguchi, S., and Sakaizumi, M., Dermal morphogenesis controls lateral line patterning during postembryonic development of teleost fish, 583-594, Copyright (2010) with permission from Elsevier. Full text @ Dev. Biol.