Fig. 7

Schematic of the hoxd9a to hoxd3a genomic region with the tested elements illustrated as described in [Fig. 4] and [Fig. 5]. The arrow color code: red, hindbrain; yellow, spinal cord; grey, somites; light blue, neuronal projection; green, pharyngeal arches. Representative larvae with GFP expression under regulatory control of CNEs that surround hoxd4a are shown at 2 to 4 dpf. The asterisk marks the level of the inner ear. d4-1. Larva showing GFP expression in the posterior spinal cord and in the somites. d4-3. GFP expression in two bilateral columns in hindbrain r2 to r6, adjacent to the inner ear. d4-4. Larva with GFP expression in spinal cord motor neurons and their projections towards the somites. Some neurons in the hindbrain r4?6 are also GFP positive. d4-6. Larva with a strong expression boundary at cerebellum/midhindbrain boundary. Strong GFP expression in the hindbrain and in the dorsal spinal cord. d4-8. Element d4-8 is located in the intron of the gene. A larva with expression in the dorsal and ventral spinal cord with GFP expressing neurons projecting towards the somites. d4-9a. Larva shows GFP expression in the hindbrain with a week anterior boundary at r6/r7. The GFP distribution continues into the spinal cord and GFP expression is also visible in the pharyngeal arches.