Fig. 8

Skeletal analyses by whole-mount staining reveal craniofacial defects and a normal ocular skeleton. Alizarin red staining, except for G, which is stained with Alcian blue. (A–C) bum^-/-, (D–F) wild-type (wt). Comparative linear dimensions are shown and indicated by a–g. (A) dorsal view, bum^-/- 30 mm SL; (B) lateral view, bum^-/- 30 mm SL; (C) dorsal view after removal of jaws, eyes and opercular complex, bum^-/- 21 mm SL; (D) dorsal view, wt 30 mm SL; (E) lateral view, wt 30 mm SL; (F) dorsal view after removal of jaws, eyes and opercular complex, wt 21 mm SL; (G) bum^-/- 10 mm SL, Alcian blue-stained; (H) scleral ossicles, bum^-/- 30 mm SL; (I) scleral ossicles of wt 21 mm SL, top, and bum^-/- 21 mm SL, below; (J) supraorbital bone of wt 21 mm SL, top, and bum^-/- 21 mm SL, below; (K) suborbital bone of wt 21 mm SL, top, and bum^-/- 21 mm SL, below; (L) lateral bones including pterygoid complex, preopercle, symplectic, and quadrate bones, wt 21 mm SL, top, and bum^-/- 21 mm SL, below; (M) hyomandibular, wt 21 mm SL, right, and bum^-/- 21 mm SL, left; (N) subopercle, wt 21 mm SL, right, and bum^-/- 21 mm SL, left; (O) interopercle, wt 21 mm SL, top, and bum^-/- 21 mm SL, below; (P) mandible, wt 30 mm SL; (Q) mandible, bum^-/- 30 mm SL; (R) mandible, bum^-/- 28 mm SL; (S) mandible, bum^-/- 30 mm SL; (T) maxilla complex, bum^-/- 30 mm SL. Abbreviations: cb, ceratobranchials; ch, ceratohyal cartilage; en, entopterygoid; mc, Meckel’s cartilage; mx, maxilla; p, palatine; pm, premaxilla; q, quadrate; sc, scleral cartilage; so, scleral ossicles; sy, symplectic. Scale bars: 500 μm unless otherwise indicated.