FIGURE

Fig. 2

ID

ZDB-FIG-100422-28

Publication

Harvey et al., 2010 - no tail integrates two modes of mesoderm induction

Other Figures

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Fig. 2

Nodal-dependent activation of ntl expression. (A,B) A transgenic line with -1 kb of the ntl promoter upstream of CFP. (A) An animal pole view shows transgene expression in the developing margin. (B) The transgene is also expressed in the developing notochord as shown at 24 hpf. (C,D) Luciferase assays performed at 6 hpf with the -2.1 kb ntl promoter fragment. (D) Wild type, -2.1 kb ntl promoter. ΔE2, -2.1 kb ntl promoter, where E2 (50 bp) was removed. ΔF, -2.1 kb ntl promoter with the putative fast-binding site (AAATATACA) removed from E2. (E) An embryo injected with a plasmid containing the -2.1 kb promoter fragment where E2 (50 bp) has been deleted (-2.1 kb ΔE2) upstream of CFP. Expression of CFP was observed in the developing margin at 6 hpf, but not in the notochord at 24 hpf.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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