FIGURE

Fig. 4

ID

ZDB-FIG-100422-20

Publication

Speirs et al., 2010 - Prostaglandin Gbetagamma signaling stimulates gastrulation movements by limiting cell adhesion through Snai1a stabilization

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Fig. 4

PGE₂ deficiency destabilizes Snai1a protein. (A) qRT-PCR of β-actin (left) and snai1a (right) transcript levels in control and ptges morphants at 60% epiboly. (B) Immunoblotting using antibodies against Snai1a (top) and α-tubulin (loading control, bottom) in control and ptges morphants. The first lane shows zebrafish embryos that were injected with snai1a-yfp (which encodes a protein of 55 kDa). (C) Confocal images of Snai1a-YFP, YFP, and membrane EGFP expression in control (top row) and ptges morphants (middle and bottom rows), and ptges morphants incubated with PGE₂ (shield stage). (D) Confocal images of ptges morphant gastrulae (shield stage) stained with anti-HA (green) and anti-E-cadherin (yellow) that were mosaically injected with snai1a-HA RNA (3 pg) at the 8- to 16-cell stage. Compare the intensity of E-cadherin staining at the membranes between two HA-positive cells (arrowhead) with that between two HA-negative cells (arrow). (E) Quantitation of E-cadherin immunostaining intensity (mean intensity) of Snai1a-HA-negative and Snai1a-HA-positive cells in ptges morphant gastrulae. ^**, P=1.16x10^-10.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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