FIGURE

Fig. 1

ID

ZDB-FIG-100304-12

Publication

Wan et al., 2010 - Negative Feedback Regulation of Wnt4 Signaling by EAF1 and EAF2/U19

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Fig. 1

Both zebrafish eaf1 and eaf2/u19 genes are downstream factors of zebrafish Wnt4a.

(A) Zebrafish embryos were injected with Wnt4-MO and wnt4a mRNA and the levels of zebrafish eaf1 mRNA determined by whole-mount in situ hybridization at 12-somite stage. (B) Zebrafish embryos were injected with Wnt4-MO and wnt4a mRNA and the levels of zebrafish eaf/u19 mRNA determined by whole-mount in situ hybridization. 12s, 12 somites. (C) Semi-quantitative RT-PCR analysis of eaf1 expression in zebrafish embryos (12 somites) injected with Wnt4a-MO and wnt4a mRNA. (D) Semi-quantitative RT-PCR analysis of eaf2/u19 expression in zebrafish embryos (12 somites) injected with Wnt4a-MO and wnt4a mRNA.

Expression Data

Genes:	eaf1 eaf2
Fish:	AB AB + MO1-wnt4
Knockdown Reagent:	MO1-wnt4
Anatomical Term:	whole organism
Stage:	10-13 somites

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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