ZFIN ID: ZDB-FIG-100107-29
Pistocchi et al., 2009 - The zebrafish prospero homolog prox1 is required for mechanosensory hair cell differentiation and functionality in the lateral line. BMC Developmental Biology   9:58 Full text @ BMC Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: Pec-fin
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Pec-fin

Fig. 5 prox1 loss of function affects development of functional hair cells in zebrafish lateral line neuromasts. Comparison of GFP expression between morphant and control embryos at 60 hpf shows no differences in mantle cells (A, B), progenitor cells (C, D) and hair cells (E, F) in different transgenic backgrounds, indicating that there is no effect of the prox1 morpholino in the specification of these cell types in the neuromast. (G, H) However, the vital stain Diasp indicates that almost 50% of the hair cells present in a neuromast are not functionally active. (I) Comparison of the percentage of GFP labeled hair cells that are co-labeled with Diasp in control and prox1 MO injected embryos. Scale bar = 10 micron.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP sqet20Et standard conditions Pec-fin unspecified IFL
sqet20Et + MO1-prox1a standard conditions Pec-fin unspecified IFL
tnks1bp1y229Gt standard conditions Pec-fin neuromast support cell IFL
tnks1bp1y229Gt + MO1-prox1a standard conditions Pec-fin neuromast support cell IFL
GFP s356tTg standard conditions Pec-fin neuromast somatic stem cell IFL
Pec-fin neuromast hair cell IFL
s356tTg + MO1-prox1a standard conditions Pec-fin neuromast somatic stem cell IFL
Pec-fin neuromast hair cell IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
s356tTg + MO1-prox1a standard conditions Pec-fin neuromast hair cell decreased amount, abnormal
Pec-fin neuromast somatic stem cell immature, abnormal
sqet20Et + MO1-prox1a standard conditions Pec-fin neuromast mantle cell morphology, normal
tnks1bp1y229Gt + MO1-prox1a standard conditions Pec-fin neuromast support cell morphology, normal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ BMC Dev. Biol.