FIGURE

Fig. S2

ID

ZDB-FIG-091214-39

Publication

Takeda et al., 2009 - DAZL relieves miRNA-mediated repression of germline mRNAs by controlling poly(A) tail length in zebrafish

Other Figures

All Figure Page

Back to All Figure Page

Fig. S2

DAZL counteracts the miRNA repression. (A) Schematic representation of the 3′UTR of GFP-IPT^miR-204 tdrd7 ΔmiR-430 site mRNA. The target site of miR-204 (blue) and the mutated site for miR-430 (gray) are shown. (B) Sequences of wildtype and mutated miR-430 target sites in the tdrd7 3′UTR. Nucleotide positions relative to the stop codon were shown above. Nucleotides that basepair with miR-430 seed are indicated in blue. (C) The GFP-IPT^miR-204 tdrd7 ΔmiR-430 site and DsRed mRNAs were injected with or without the mRNA encoding Myc-DAZL at the one-cell stage. Subsequently, the miR-204 duplex was injected at the two-cell stage. GFP and DsRed were analyzed at 24 hpf. Fold change of normalized GFP fluorescence relative to mock control is shown on the right.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One