Fig. 1
- ID
- ZDB-FIG-091214-33
- Publication
- Takeda et al., 2009 - DAZL relieves miRNA-mediated repression of germline mRNAs by controlling poly(A) tail length in zebrafish
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Activation of GFP-tdrd7 expression by DAZL. (A) GFP-tdrd7 and DsRed mRNAs were co-injected with the mRNAs encoding Myc-tagged DAZL, DAZL F91A, DAZL RRM, or DAZL ΔDAZ mRNA, or without any dazl mRNA (mock) at the one-cell stage. GFP and DsRed expression were analyzed at 24 hr post-fertilization (hpf). Fold change of normalized GFP fluorescence relative to mock control is shown on the right. (B) In situ hybridization of the injected embryos at 80% epiboly with an antisense probe for GFP. GFP-tdrd7 and DsRed mRNAs were injected with or without the mRNA encoding Myc-DAZL. (C) Northern blotting of the injected GFP-tdrd7 and DsRed mRNAs. The ratio of GFP mRNA to DsRed mRNA from three independent experiments is shown. |