FIGURE

Fig. 2

ID

ZDB-FIG-091007-3

Publication

Jacoby et al., 2009 - The zebrafish dystrophic mutant softy maintains muscle fibre viability despite basement membrane rupture and muscle detachment

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Fig. 2

Muscle degeneration in sof mutants results from detachment of myofibres from the vertical myoseptum. Lateral views of wild-type muscle (A,C,E) show fibres spanning the somite between adjacent myosepta, whereas in sof mutants (B,D,F), lesions rapidly develop. (A,B) Whole-mount immunohistochemistry using an antibody against slow myosin demonstrates that, although slow fibres develop normally in sof embryos, by 30 hpf fibres have already detached from the myoseptum. (C-F) DIC images of axial muscle. By 72 hpf, the sof embryo appears severely damaged, with numerous detached fibres scarring the myotome. A magnified view of a single detached fibre (F) shows the characteristic invaginated membrane (arrows) and retraction groove (arrowhead). (G) Retraction of a single fibre (arrowhead), from left of frame to right, in a 72 hpf sof embryo. Still images taken from Movie 1 in the supplementary material, with elapsed time indicated in seconds.

Expression Data

Antibody:	Ab-F59
Fish:	WT lamb2^{tm272a/tm272a}
Anatomical Term:	slow muscle cell
Stage:	Prim-15

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	lamb2^{tm272a/tm272a}
Observed In:	muscle attachment myotome muscle cell slow muscle cell
Stage Range:	Prim-15 to Protruding-mouth

Phenotype Detail

Acknowledgments

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