Fig. 6
Ptf1a:GFP amacrine cells at 120 hpf have stable neurite arbors. Single time-lapse images of different amacrine cells imaged for up to 24 hours. The GFP signal is relatively weak in some cells but, nonetheless, the stratification depth extent and lateral neurite arbor width remain unchanged over the imaging period. In some brighter labelled cells, even individual branches or varicosities are seen to remain stable (white arrowheads), in contrast to the rapid neurite remodelling of amacrine cells, which has been previously described up till 73 hpf [29]. Scale bar = 20 μm. |