Fig. 2

Comparison of the embryonic expression patterns of hip and ptc1. Images of whole-mount preparations hybridised with riboprobe to hip or ptc1. hip and ptc1 expression domains have similarities, e.g., in the adaxial region (ad) of the somites (s) (A–D and Q, R), but are not identical. In particular, note the differences in forebrain expression between C and D (arrowheads) and F and I, and the hindbrain (hb) expression present in J (hip; arrowhead) but not in G (ptc1). In addition, in the neural tube, hip is expressed in only a subset of the ptc1 domain: compare L (hip) and P (ptc1). Note that in the head, the posterior domain of a region of strong neural tube expression lies adjacent to the anterior part of the otic vesicles from 18hpf (18S) to 26hpf (E, L; arrow in panel E): dotted lines demarcate the left otic vesicle in panels E, L, P. Other regions of hip expression include the fin buds, branchial arches, pronephros and mouth region. (A–D) Lateral views; anterior to top. (F, G, I–K) Lateral views; anterior to left, dorsal to top. (H) Hand cut transverse section at anterior limit of embryo showing mouth region. All other panels: dorsal views, anterior to left. Abbreviations: ad, adaxial cells; ba, branchial arches; f, fin buds; hb, hindbrain; hy, hypothalamus; lf, lateral fibres; l, lens; m, mouth; nt, neural tube; otv, otic vesicle; p, pronephros; s, somites; t, telencephalon; te, tectum; zli, zona limitans intrathalamica. Panels J and Q are composites of more than one image.