Fig. 1

Persistent formation of vascular plexuses in ontogenetic and regenerating blood vessels in zebrafish prp mutants. (A) Wild-type (WT) and (B) prp adult zebrafish showing that the prp mutant has a normal body but smaller fins with rounded edges (arrows). (C) to (F) Regenerating experiments of fish after amputation. (C) A representative 3-day post-amputation (dpa) regenerating fin ray of the WT showing the vascular plexus at the fin tip (double-headed arrow). (D) Vascular plexuses seemed to form normally in prp 3-dpa regenerating fins (double-headed arrow). (E) At 10 dpa, fin vessels continued to regenerate without forming a plexus in the WT. (F) In the prp mutant, vascular plexuses (double-headed arrows), however, were still present in the 10-dpa regenerating fins. (G, H) WT and prp mutant fish. (G) The vascular plexus is normally not seen at the tips of normal WT fins. (H) In contrast, at almost every tip of a normal prp fin, a plexus-like vascular network could easily be detected (arrows).