FIGURE

Fig. 3

ID

ZDB-FIG-090804-21

Publication

Abbas et al., 2009 - Nkcc1 (Slc12a2) is required for the regulation of endolymph volume in the otic vesicle and swim bladder volume in the zebrafish larva

Other Figures

All Figure Page

Back to All Figure Page

Fig. 3

Expression of ionic homeostasis genes is disrupted in the ears of lte mutants. (A-H) Whole-mount in situ hybridisation showing that expression of alpha (a) and beta (b) Na⁺/K⁺-ATPase subunit genes is downregulated in the inner ear of lte mutants at 74 hpf (atp1b1a in E,F; atp1b2b in G,H), 4 dpf (atp1a1a.4 in A,B) and 5 dpf (atp1a1a.5 in C,D). The endolymphatic duct (asterisk, A,B) expresses atp1a1a.4 and appears normal in lte. (I,J) At 4 dpf, otic expression of the potassium channel gene kcnq1 is also reduced, mainly in the central semicircular canal hub (h). (K,L) Conversely, expression of connexin 33.8 (cx33.8) at 5 dpf is expanded in the anterior macula of lte mutants (black arrowheads), but remains unchanged in the lateral crista (white arrowheads; out of plane of focus in K). Scale bar: 50 μm.

Expression Data

Genes:	atp1a1a.4 atp1a1a.5 atp1b1a atp1b2b gjb8 kcnq1.1
Fish:	WT slc12a2^to27d/to27d
Anatomical Terms:	anterior macula lateral crista saccule semicircular canal
Stage Range:	Protruding-mouth to Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	slc12a2^to27d/to27d
Observed In:	anterior macula inner ear inner ear morphogenesis lateral crista semicircular canal
Stage Range:	Protruding-mouth to Day 5

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development