FIGURE

Fig. 4

ID

ZDB-FIG-090804-16

Publication

Yamauchi et al., 2009 - Fgf4 is required for left-right patterning of visceral organs in zebrafish

Other Figures

All Figure Page

Back to All Figure Page

Fig. 4

The formation of Kupffer′s vesicle in Fgf4 knockdown embryos. Kupffer′s vesicle in Fgf4 MO1-injected embryos at 12 hpf was morphologically normal (100%, n = 32) (a, b). The expression of lrdr1, which is essential for cilia to function in Kupffer′s vesicle, was examined by whole mount in situ hybridization. The expression was also unaffected in Fgf4 MO1-injected embryos at 10 hpf (93.3%, n = 30) (c, d). (a–d) Dorsal views of the tail bud. A scale bar = 200 μm. Cilia exist on the cells in Kupffer′s vesicle. The expression of acetylated tubulin, a marker protein for cilia, was examined by immunohistochemistry using an anti-acetylated tubulin antibody. The size of Kupffer′s vesicle and the number of cilia of Kupffer′s vesicle in Fgf4 MO-injected embryos were not significantly affected (100%, n = 30); however, the average length of cilia of Kupffer′s vesicle in Fgf4 MO-injected embryos (3.27 ± 1.06 μm, n = 204) was significantly shorter than that in wild-type embryos (4.41. ± 0.91 μm, n = 214) (e, f). These results indicate that Fgf4 is not essential for the morphology and function of Kupffer′s vesicle. A scale bar = 20 μm.

Expression Data

Gene:	dnah9
Fish:	WT
Anatomical Term:	forerunner cell group
Stage:	Bud

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO3-fgf4
Knockdown Reagent:	MO3-fgf4
Observed In:	Kupffer's vesicle motile cilium
Stage:	5-9 somites

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 332(1), Yamauchi, H., Miyakawa, N., Miyake, A., and Itoh, N., Fgf4 is required for left-right patterning of visceral organs in zebrafish, 177-185, Copyright (2009) with permission from Elsevier. Full text @ Dev. Biol.