Fig. S4

The neural plate border develops independent of foxd3 and tfap2a function. (A) pax3 expression at the 3 somite stage, dorsal views with anterior to the top. paired homeobox 3 (pax3) is expressed at the edge of the developing neural plate, and is required for induction of NC-specific gene expression. pax3 is robustly expressed in foxd3^zdf10;tfap2a^low double mutants, as well as in foxd3^zdf10 and tfap2a^low single mutant embryos. (B) msxb expression at the 3 somite stage, dorsal views with anterior to the top. msxb expression is required for proper size and positioning of the neural plate border. msxb expression is normal in foxd3^zdf10;tfap2a^low double mutant, foxd3^zdf10 single mutant and tfap2a^low single mutant embryos compared with wild-type siblings. (C) huC expression in 6 somite stage embryos. At this stage, primary motor neurons (more medial cells) and Rohon-Beard sensory neurons (bilateral stripes of cells just lateral to primary motorneurons) express huC. Both neuronal populations develop normally in both single mutants, as well as in double mutants.