Fig. 7
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- ZDB-FIG-090527-45
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- North et al., 2009 - Hematopoietic stem cell development is dependent on blood flow
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The Effect of NO Signaling on HSC Development in the AGM Is Conserved in Mice (A–H) FACS analysis of dissociated AGM cells in WT and Nos KO mice at e11.5. Nos3-/- mice exhibited a decrease in the Sca1+/cKit+ and CD45+/VE-Cadherin+ populations, while deletion of Nos1 had no significant effects. (I–L) Histological sections through the AGM region of e11.5 embryos; the inset represents a high-magnification view around the hematopoietic clusters. L-NAME exposure causes absence of hematopoietic clusters; Nos3-/- mice exhibit smaller cluster size, while Nos1-/- does not impair cluster formation. Serial sections through the entire aorta of at least ten embryos per genotype/treatment were analyzed. (M and N) Effect of NO signaling on AGM HSC function. AGM regions of somite stage-matched WT, L-NAME treated or Nos3-/- progeny were subdissected at e11.5 and transplanted into sublethally irradiated recipients. L-NAME exposure or Nos3 deletion in embryos significantly reduced CFU-S12 spleen colony formation (mean ± SD; * sig versus control; p < 0.001; ** sig versus L-NAME; p < 0.05; ANOVA, n ≥ 5) (M). Diminished NO signaling significantly decreased embryonic donor cell chimerism rates in individual recipient mice at 6 weeks after transplant (* sig versus control, p < 0.05, ANOVA, n ≥ 5) (N). |
Reprinted from Cell, 137(4), North, T.E., Goessling, W., Peeters, M., Li, P., Ceol, C., Lord, A.M., Weber, G.J., Harris, J., Cutting, C.C., Huang, P., Dzierzak, E., and Zon, L.I., Hematopoietic stem cell development is dependent on blood flow, 736-748, Copyright (2009) with permission from Elsevier. Full text @ Cell