ZFIN ID: ZDB-FIG-090511-10
Castets et al., 2009 - Inhibition of Endothelial Cell Apoptosis by Netrin-1 during Angiogenesis. Developmental Cell   16(4):614-620 Full text @ Dev. Cell
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Prim-5 to Long-pec

Fig. 3 Phenotype of Knockdown netrin-1a Zebrafish Embryos Is Rescued by Inhibition of Apoptosis

(A–C) fli:egfp zebrafish embryos were injected at the one- to four-cell stage with control (ctl), splice (spl), or translation (tsl)-blocking netrin-1a, UNC5B, and DAP kinase morpholinos, alone or in combination. Phenotypes were analyzed from 24 to 54 hours postfertilization. Representative images of trunk vasculature are shown. Anterior is oriented toward the left. (A and B) The caspase inhibitor BAF restores parachordal vessel formation (asterisks) in (A) zebrafish embryos injected with spl-blocking netrin-1a morpholinos and intersegmental vessel formation in (B) zebrafish embryos injected with netrin-1a tsl morpholinos. (B) Similar phenotypical rescue was observed by simultaneous injection of UNC5B or DAP kinase and tsl-blocking netrin-1a morpholinos in zebrafish embryos. (A and B) Quantification is presented; p values are indicated (χ2 test). (C) BAF treatment or injection of UNC5B-blocking morpholinos inhibit the increase in apoptosis observed after injection of tsl-blocking netrin-1a morpholinos in zebrafish embryos, as shown by TUNEL staining (left panels) or caspase-3 activity (right panel). Caspase-3 activity is expressed as a relative index referred to embryos injected with netrin-1a tsl morpholinos solely. Means and SEM are calculated from three independent experiments; p values are indicated (Student t′s test).

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
y1Tg + MO1-ntn1a standard conditions Prim-5 apoptotic process increased occurrence, abnormal
Prim-5 embryo development disrupted, abnormal
Prim-5 intersegmental vessel disorganized, abnormal
Prim-5 whole organism lacks all parts of type dorsal longitudinal anastomotic vessel, abnormal
Prim-5 whole organism lacks parts or has fewer parts of type intersegmental vessel, abnormal
y1Tg + MO1-ntn1a + MO1-unc5b standard conditions Prim-5 apoptotic process occurrence, normal
Prim-5 whole organism has fewer parts of type intersegmental vessel, abnormal
y1Tg + MO3-ntn1a standard conditions Long-pec dorsal longitudinal anastomotic vessel physical object quality, normal
Long-pec intersegmental vessel physical object quality, normal
Long-pec whole organism lacks parts or has fewer parts of type parachordal vessel, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Cell for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Cell, 16(4), Castets, M., Coissieux, M.M., Delloye-Bourgeois, C., Bernard, L., Delcros, J.G., Bernet, A., Laudet, V., and Mehlen, P., Inhibition of Endothelial Cell Apoptosis by Netrin-1 during Angiogenesis, 614-620, Copyright (2009) with permission from Elsevier. Full text @ Dev. Cell