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General Information
ZIRC
ZFIN ID: ZDB-FIG-090501-64
de Pater et al., 2009 - Distinct phases of cardiomyocyte differentiation regulate growth of the zebrafish heart. Development (Cambridge, England)   136(10):1633-1641 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Term:
Stage: Long-pec
PHENOTYPE:
Fish:
Observed In:
Stage: Long-pec

Fig. 5 Reduced cardiomyocyte differentiation at the venous pole in isl1 mutant embryos. (A-F) Single z-scan of the atrium of a representative isl1K88X sibling (A-C) and mutant (D-F) embryo. White arrow (B) indicates eGFPposDsRedneg cardiomyocytes at the venous pole of the wild-type sibling heart; red arrow (E) indicates the venous pole of the isl1 mutant where only very few eGFPposDsRedneg cells are present. (G,H) Single z-scan of a confocal image at the level of the arterial pole showing the eGFPposDsRedneg cardiomyocytes in the isl1 mutant (white arrow). The region shown is similar to that of the control embryo shown in Fig. 2G-I. V, ventricle: A, atrium. Scale bar: 50 μm. (I) Schematic of the location of the eGFPposDsRedpos cells (yellow) and the eGFPposDsRedneg (green) cells in the arterial pole of the isl1 mutant. (J,K) The number of eGFPposDsRedpos (J) and eGFPposDsRedneg (K) cardiomyocytes per embryo. (K) The eGFPposDsRedneg cardiomyocytes are subdivided into eGFPposDsRedneg cells being present at either the arterial or the venous pole. Note the significant reduction of eGFPposDsRedneg cells at the venous pole in the isl1 mutants. Bars represent mean±s.e.m. **P<0.01.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
DsRed2 f2Tg/+; twu26Tg/+ standard conditions Long-pec heart IFL
isl1sa29/sa29; f2Tg/+; twu26Tg/+ standard conditions Long-pec heart IFL
EGFP f2Tg/+; twu26Tg/+ standard conditions Long-pec heart IFL
isl1sa29/sa29; f2Tg/+; twu26Tg/+ standard conditions Long-pec heart IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
isl1sa29/sa29; f2Tg/+; twu26Tg/+ standard conditions Long-pec atrial cardiac muscle cell differentiation disrupted, abnormal
Long-pec atrium cardiac muscle cell decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development