PUBLICATION
Distinct phases of cardiomyocyte differentiation regulate growth of the zebrafish heart
- Authors
- de Pater, E., Clijsters, L., Marques, S.R., Lin, Y.F., Garavito-Aguilar, Z.V., Yelon, D., and Bakkers, J.
- ID
- ZDB-PUB-090429-10
- Date
- 2009
- Source
- Development (Cambridge, England) 136(10): 1633-1641 (Journal)
- Registered Authors
- Bakkers, Jeroen, Yelon, Deborah
- Keywords
- Fgf, Differentiation, Heart, Islet1, Zebrafish
- MeSH Terms
-
- Animals
- Cell Differentiation/physiology*
- Cell Proliferation
- Fibroblast Growth Factors/physiology
- Heart/embryology*
- Heart/physiology
- Myocytes, Cardiac/cytology*
- Myocytes, Cardiac/physiology
- Signal Transduction/physiology
- Zebrafish/embryology*
- Zebrafish/physiology
- PubMed
- 19395641 Full text @ Development
Citation
de Pater, E., Clijsters, L., Marques, S.R., Lin, Y.F., Garavito-Aguilar, Z.V., Yelon, D., and Bakkers, J. (2009) Distinct phases of cardiomyocyte differentiation regulate growth of the zebrafish heart. Development (Cambridge, England). 136(10):1633-1641.
Abstract
Amongst animal species, there is enormous variation in the size and complexity of the heart, ranging from the simple one-chambered heart of Ciona intestinalis to the complex four-chambered heart of lunged animals. To address possible mechanisms for the evolutionary adaptation of heart size, we studied how growth of the simple two-chambered heart in zebrafish is regulated. Our data show that the embryonic zebrafish heart tube grows by a substantial increase in cardiomyocyte number. Augmented cardiomyocyte differentiation, as opposed to proliferation, is responsible for the observed growth. By using transgenic assays to monitor developmental timing, we visualized for the first time the dynamics of cardiomyocyte differentiation in a vertebrate embryo. Our data identify two previously unrecognized phases of cardiomyocyte differentiation separated in time, space and regulation. During the initial phase, a continuous wave of cardiomyocyte differentiation begins in the ventricle, ends in the atrium, and requires Islet1 for its completion. In the later phase, new cardiomyocytes are added to the arterial pole, and this process requires Fgf signaling. Thus, two separate processes of cardiomyocyte differentiation independently regulate growth of the zebrafish heart. Together, our data support a model in which modified regulation of these distinct phases of cardiomyocyte differentiation has been responsible for the changes in heart size and morphology among vertebrate species.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping