FIGURE

Fig. 2

ID

ZDB-FIG-090415-11

Publication

Raz et al., 1998 - �-lactamase as a marker for gene expression in live zebrafish embryos

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Fig. 2

Cell-autonomous detection of the β-lactamase marker. (A) One-cell-stage embryos were injected with CCF2 and later at the 16-cell stage one of the marginal blastomeres was injected with β-lactamase mRNA. At this stage the cytoplasm of the marginal blastomeres is not separated allowing the mRNA to diffuse and to be translated in more than 1/16 of the cells. (B, C) One-cell-stage embryos were injected with CCF2 and later at the 64-cell stage one of the blastomeres was coinjected with β-lactamase mRNA and with rhodamine dextran as a lineage marker. At 30% epiboly the yolk was removed and a flat preparation of the embryo was visualized at a high magnification using the CCF2 filter set (B) or with a filter set for rhodamine detection (C).

Expression Data

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Antibody Labeling

Phenotype Data

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Acknowledgments

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Reprinted from Developmental Biology, 203, Raz, E., Zlokarnik, G., Tsien, R.Y., and Driever, W., �-lactamase as a marker for gene expression in live zebrafish embryos, 290-294, Copyright (1998) with permission from Elsevier. Full text @ Dev. Biol.