ZFIN ID: ZDB-PUB-981208-28
ß-lactamase as a marker for gene expression in live zebrafish embryos
Raz, E., Zlokarnik, G., Tsien, R.Y., and Driever, W.
Date: 1998
Source: Developmental Biology 203: 290-294 (Journal)
Registered Authors: Driever, Wolfgang, Raz, Erez
Keywords: zebrafish; ß-lactamase; dominant marker
MeSH Terms: Animals; Biomarkers*; Fluoresceins/metabolism; Fluorescence; Fluorescent Dyes/metabolism (all 14) expand
PubMed: 9808780 Full text @ Dev. Biol.
FIGURES   (current status)
In this report we describe the development of a sensitive assay for gene expression in zebrafish embryos using beta-lactamase as a reporter gene. We show that injection of a green fluorescent substrate for beta-lactamase allows the detection of reporter gene expression in live embryos. The beta-lactamase enzyme catalyzes the hydrolysis of the substrate, thereby disrupting fluorescence resonance energy transfer from the donor to the acceptor dye in the molecule. As a result, a blue fluorescent product is produced and retained specifically in cells within which the enzyme is expressed. beta-Lactamase is therefore suitable for monitoring spatially restricted patterns of gene expression in the early embryo. We suggest that this new reporter system provides a major advancement in sensitivity over the existing methods for monitoring gene expression in vivo during early embryogenesis.