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Fig. S6

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ZDB-FIG-090325-9

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Goessling et al., 2009 - Genetic interaction of PGE2 and Wnt signaling regulates developmental specification of stem cells and regeneration

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Fig. S6

PGE2 enhances wnt activity in the HSC compartment via the PKA/cAMP pathway
(A and B) qPCR results for runx1 expression after wnt8 transgene induction and chemical treatments. * statistically significant vs. control; ** statistically significant vs. wnt8 (A) or dmPGE2 (B), ANOVA, p<0.001; n=3 sets of 50 embryos; data represented as mean ± SD.
(A) cAMP stimulation by forskolin (0.5μM) and wnt8 induction enhanced runx1 levels at 36 hpf in qPCR analysis of whole embryo lysates. Forskolin improved the negative effects of indomethacin treatment in both controls and wnt8 transgenics.
(B) PKA inhibition by H89 (0.5μM) reduced runx1 expression by qPCR in both control and dmPGE2 treated embryos.
(C and D) dmPGE2 (10μM) enhanced runx1/cmyb expression in the zebrafish tail at 36 hpf.
(E and F) PKA inhibition by KT5720 (1μM) had similar effects on HSC number in wildtype and dmPGE2-treated embryos as seen with H89 exposure.
(G and H) PI3K inhibition by LY294002 (1μM) or (I and J) Wortmannin (1μM) did not affect HSC number in wild-type or dmPGE2-treated embryos.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Cell, 136(6), Goessling, W., North, T.E., Loewer, S., Lord, A.M., Lee, S., Stoick-Cooper, C.L., Weidinger, G., Puder, M., Daley, G.Q., Moon, R.T., and Zon, L.I., Genetic interaction of PGE2 and Wnt signaling regulates developmental specification of stem cells and regeneration, 1136-1147, Copyright (2009) with permission from Elsevier. Full text @ Cell