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Fig. 4

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ZDB-FIG-090325-4

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Goessling et al., 2009 - Genetic interaction of PGE2 and Wnt signaling regulates developmental specification of stem cells and regeneration

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Fig. 4

PGE2 Regulates the Effects of wnt Activity on HSCs via cAMP/PKA Signaling

(A, B, E, F, I, J, M, and N) The /PGE2/wnt interaction affected runx1/cmyb+ HSC formation, as seen in Figure 2. (C and G) cAMP enhancement by forskolin increased runx1/cmyb expression in WT embryos and further expanded HSCs in wnt8 embryos. (D and H) Forskolin counteracted the inhibitory effect of indo on HSC formation in WT and wnt8 embryos. (K and O) PKA inhibition by H89 decreased runx1/cmyb expression in WT embryos and eradicated HSC formation in dkk embryos. (L and P) The enhancing effect of dmPGE2 on HSCs was reduced back to baseline levels by PKA inhibition; similarly, the dmPGE2-induced rescue of HSCs in dkk embryos was blocked by H89.

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Reprinted from Cell, 136(6), Goessling, W., North, T.E., Loewer, S., Lord, A.M., Lee, S., Stoick-Cooper, C.L., Weidinger, G., Puder, M., Daley, G.Q., Moon, R.T., and Zon, L.I., Genetic interaction of PGE2 and Wnt signaling regulates developmental specification of stem cells and regeneration, 1136-1147, Copyright (2009) with permission from Elsevier. Full text @ Cell