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ZIRC
ZFIN ID: ZDB-FIG-090127-69
Tu et al., 2009 - Nkx2.7 and Nkx2.5 function redundantly and are required for cardiac morphogenesis of zebrafish embryos. PLoS One   4(1):e4249 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: Prim-25 to Protruding-mouth
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Prim-25 to Protruding-mouth

Fig. 1 The defective phenotypes of zebrafish embryo heart injected with Nkx2.5-MO, Nkx2.7-MO and Nkx2.5/2.7-MO.

Eight nanograms of MO were injected into one-cell stage embryos derived from transgenic line Tg (cmlc2::GFP) to knock down the Nkx protein specifically. The embryos are shown at 36 hpf (A, B, C), 48 hpf (D, E, F), and 72 hpf (G, H, I). The heart phenotype of Nkx2.5-MO embryos was similar to that of control embryos whose ventricle is located at the right side of the atrium when embryos were observed at 36 hpf, 48 hpf and 72 hpf from the ventral view under fluorescence microscope (A, D, G). However, embryos injected with Nkx2.7-MO displayed an unlooping defect from 36 hpf to 72 hpf (B, E, H). Embryos injected with Nkx2.5/2.7-MO displayed a shrunken ventricle and an expanding atrium (C, F, I). v: ventricle; a: atrium.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP twu34Tg control Prim-25 presumptive atrium heart tube IFL
Prim-25 presumptive cardiac ventricle heart tube IFL
Long-pec atrium IFL
Long-pec cardiac ventricle IFL
Protruding-mouth atrium IFL
Protruding-mouth cardiac ventricle IFL
twu34Tg + MO2-nkx2.5 + MO3-nkx2.7 standard conditions Prim-25 presumptive atrium heart tube IFL
Prim-25 presumptive cardiac ventricle heart tube IFL
Long-pec atrium IFL
Long-pec cardiac ventricle IFL
Protruding-mouth atrium IFL
Protruding-mouth cardiac ventricle IFL
twu34Tg + MO3-nkx2.7 standard conditions Prim-25 presumptive atrium heart tube IFL
Prim-25 presumptive cardiac ventricle heart tube IFL
Long-pec atrium IFL
Long-pec cardiac ventricle IFL
Protruding-mouth atrium IFL
Protruding-mouth cardiac ventricle IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
twu34Tg + MO2-nkx2.5 + MO3-nkx2.7 standard conditions Prim-25 - Protruding-mouth atrium distended, abnormal
Prim-25 - Protruding-mouth cardiac ventricle hypotrophic, abnormal
Prim-25 - Protruding-mouth heart development disrupted, abnormal
Prim-25 - Protruding-mouth heart looping disrupted, abnormal
twu34Tg + MO3-nkx2.7 standard conditions Prim-25 - Protruding-mouth heart development disrupted, abnormal
Prim-25 - Protruding-mouth heart looping disrupted, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One