FIGURE

Fig. 7

ID

ZDB-FIG-090113-27

Publication

Matsuda et al., 2009 - Interaction with Notch determines endocytosis of specific Delta ligands in zebrafish neural tissue

Other Figures

All Figure Page

Back to All Figure Page

Fig. 7

Mib1, Notch1a and Notch3 are not essential for DeltaC endocytosis. (A-C′) Distribution of DeltaC in wild-type zebrafish embryos (A,A′) and in two mib alleles, mib^m178 (B,B′) and mib^ta5ab (C,C′). (D,D′) Distribution of DeltaC in Notch MO embryos. Eight-somite stage embryos were double stained with zebrafish DeltaC antibody zdC1 (grey in A-D, green in A′-D′) and β-catenin (red in A′-D′). A dorsal view of a lateral neurogenic domain in the caudal neural plate is shown. In WT embryos, only subset of cells express DeltaC in cytoplasmic puncta (A,A′). A neurogenic phenotype allows more cells to express DeltaC in both mib mutants and Notch MO embryos as Notch signaling is reduced (B-D). However, DeltaC distribution remains cytoplasmic in mib^m178 (B,B′) and Notch MO embryos (D,D′), and it only accumulates on the cell surface in mib^ta52b mutant embryos (C,C′).

Expression Data

Antibodies:	Ab1-dlc Ab3-ctnnb
Fish:	WT mib1^m178/m178 mib1^ta52b/ta52b WT + MO1-notch1a + MO1-notch3
Knockdown Reagents:	MO1-notch1a MO1-notch3
Anatomical Terms:	hindbrain neural plate adherens junction hindbrain neural plate cell surface hindbrain neural plate cytoplasm
Stage:	5-9 somites

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development