FIGURE

Fig. 6

ID

ZDB-FIG-090112-13

Publication

Meng et al., 2008 - Targeting retinal dopaminergic neurons in tyrosine hydroxylase-driven green fluorescent protein transgenic zebrafish

Other Figures

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Fig. 6

Analysis of two subpopulations of GFP-positive cells using single-cell RT–PCR. A: Representative bright and dim GFP-positive cells in dispersed retinal culture were imaged at the same excitation intensity. Scale bar equals 10 μm. B: Schematic diagram shows the primers used for seminested PCR. For th, the size of the 2nd round PCR product was 277 bp. For dat, the size was 250 bp. C: Representative gel image shows the 2nd round PCR product from dim GFP cells, bright GFP cells, and medium control sample. Primer sets used were indicated at the bottom. D: Expression frequency of th and dat genes is different between bright GFP expressing cells and dim GFP expressing cells as assayed by single-cell RT–PCR. For bright GFP-expressing cells, 8 cells were examined. For dim GFP-expressing cells, 7 cells were examined.

Expression Data

Gene:	nelfcd
Fish:	m899Tg/+ (AB)
Anatomical Term:	retina
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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