Fig. 2

Temporal expression of ZF-RNases-1, -2 and -3 genes in zebrafish late embryos and larvae. Zebrafish late embryos and larvae stages were analyzed by reverse transcriptase followed by PCR (RT-PCR). A, Only ZF-RNases-1 is detected in late embryos and all larvae stages analyzed. In contrast, neither ZF-RNases-2, nor ZF-RNases-3 gene expression is observed at these stages. The -RT lane is a negative control for the reverse transcription reaction. ZF-Odc is a loading control. B, Quantitative analysis of ZF-RNases-1 gene performed by real-time PCR. These data correlate with the above results showing the highest expression level of ZF-RNases-1 at 24 hpf. C, Quantitative real-time PCR (40 cycles) performed on late embryos and larvae stages of ZF-RNase-2 at 13 dpf stage. D, Quantitative real-time PCR (40 cycles) on late embryos and larvae stages for ZF-RNase-3 expression in 13 dpf larvae. Quantified mRNA values were normalized by the amounts of ZF-Odc mRNA, the house keeping gene, and results are given as fold induction.