FIGURE

Fig. 3

ID

ZDB-FIG-081230-9

Publication

Ninkovic et al., 2008 - Gsk3{beta}/PKA and Gli1 regulate the maintenance of neural progenitors at the midbrain-hindbrain boundary in concert with E(Spl) factor activity

Other Figures

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Fig. 3

Activated PKA can compensate for reduced Gsk3β or E(Spl) activity to promote IZ formation. Zebrafish embryos analyzed at the 3- to 5-somite stage for neurog1 and pax2.1 and flat-mounted, anterior up. Bracket, MIZ; arrows indicate ectopic neurog1 expression. (A-D) Activated PKA (PKA^*, injected as capped RNA at the one-cell stage) does not affect IZ formation (compare A with D, see also F) and blocks LiCl-induced neurog1 expression (arrows) across the MIZ (compare B with C). (E-H) Coinjection of PKA^* RNA and her5GripNA (G) blocks the neurogenic effect of her5GripNA alone (E, arrows) and restores the MIZ. (H) Control.

Expression Data

Genes:	neurog1 pax2a
Fish:	AB
Condition:	chemical treatment: pharmaceutical
Anatomical Term:	presumptive intervening zone
Stage:	1-4 somites

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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