FIGURE

Fig. 3

ID

ZDB-FIG-081222-3

Publication

Montpetit et al., 2008 - Disruption of AP1S1, causing a novel neurocutaneous syndrome, perturbs development of the skin and spinal cord

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Fig. 3

Ap1s1 knockdown is associated with abnormal distribution of laminin and cadherin.

Despite changes in the size and shape of the tail, p63-labelled nuclei (orange) of basal keratinocytes were present in KD larvae. (B). The insets show enlarged views of small groups of keratinocytes. Immunolabelling for laminin (green) in WT (C) showed normal distribution of the basement membrane along the fin fold margin, whereas in the KD larvae the residual laminin labeling was diffuse and disorganized (D). Compared to WT keratinocytes (E) labeled with p63 (orange), cadherin (green) is decreased at the cell membrane of the KD larvae (F). However, the localization of cytokeratin in WT (G) and in KD larvae (H) appeared similar. Scale bars: 10 μm, Insets in A, B, = 20 μm.

Expression Data

Antibodies:	Ab1-lama1 Ab1-pan-Cadherin Ab1-tp63
Fish:	WT WT + MO1-ap1s1
Knockdown Reagent:	MO1-ap1s1
Anatomical Terms:	caudal fin basement membrane caudal fin plasma membrane keratinocyte
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-ap1s1
Knockdown Reagent:	MO1-ap1s1
Observed In:	caudal fin basement membrane
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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