FIGURE

Fig. 6

ID

ZDB-FIG-081219-40

Publication

Berndt et al., 2008 - Rho-kinase and myosin II affect dynamic neural crest cell behaviors during epithelial to mesenchymal transition in vivo

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Fig. 6

Inhibition of myosin II or ROCK disrupts blebbing and causes defects in NCC EMT. (A–J) Time-lapse sequences of cells expressing GPI-GFP (membranes, blue) and RFP-UtrCH (F-actin, red) in vehicle treated (DMSO) (A–E) or 50 μM blebbistatin-treated (F–J) embryos. Arrowheads in A, C indicate normal blebs. Arrowhead in F indicates bleb that fails to retract after blebbistatin treatment. Dorsal view adjacent to rhombomere 2, anterior is right. Time is in seconds. (K–N) In situ hybridization for dlx2 in embryos treated with DMSO (K), or 10 μM (L) or 50 μM (M) blebbistatin, or 50 μM ROCK inhibitor (N). Dorsal views, anterior left. (O) Number of cells undergoing EMT per hour during 2 h movies. Blebbistatin (BBS) and ROCKi were at 50 μM. Scale bar = 10 μm for A–J and 40 μm for K–N.

Expression Data

Gene:	dlx2a
Fish:	WT
Conditions:	chemical treatment: blebbistatin chemical treatment: pharmaceutical
Anatomical Term:	neural crest
Stage:	14-19 somites

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 324(2), Berndt, J.D., Clay, M.R., Langenberg, T., and Halloran, M.C., Rho-kinase and myosin II affect dynamic neural crest cell behaviors during epithelial to mesenchymal transition in vivo, 236-244, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.