hgf1 and hgf2 expression from the onset of gastrulation through 72 hr. A-C: Whole-mount, dorsal views of embryos probed for hgf1 expression. The animal pole is to the top. A: Shield-stage (6 hr). B: Ninety percent epiboly (9 hr) embryos express hgf1 in a subset of dorsal cells (arrow). C: By 10.5 hr (1 somite-stage), adaxial cells express hgf1 (arrowhead) as well as deep anterior cells (inset, arrow). D: Whole-mount lateral view at 16.5 hr (15 somite-stage) showing hgf1 expression in the somites (arrow). E,F: Whole-mount, 36-hr embryos. E: hgf1 expression in the pharyngeal arches (arrowhead) and somites (arrow) is visible in lateral view. F: Dorsal view showing pharyngeal arch (arrowhead) and early swim bladder hgf1 expression (arrow). Fin bud expression (asterisk) is visible just posterior to the arches. G-I: Forty-eight hours. G: Lateral view showing pharyngeal arch (arrowhead) and fin bud (asterisk) hgf1 expression. H: Dorsal view showing pharyngeal arch (arrowhead), swim bladder (arrow), and fin bud (asterisk) hgf1 expression. I: Transverse section at the level of the swim bladder showing hgf1 expression in cells surrounding the swim bladder (arrow). The neural tube (NT) is marked for reference. J: Whole-mount dorsal view at 72 hr showing pharyngeal arch (arrowhead), fin bud (asterisk), and swim bladder (arrow) hgf1 expression. Note lack of liver staining near the left fin bud. K: Whole-mount, dorsal view at 10.5 hr (1 somite-stage) showing lack of hgf2 expression at this stage (embryo has been overstained). The embryonic midline is marked with an arrow. L,M: Embryos (16.5 hr, 15 somite-stage) showing hgf2 expression in lateral (L) and dorsal views (M). Pharyngeal arch (arrows) and otic vesicle (arrowheads) expression is visible at this stage. Note lack of somite expression. N: Thirty-six-hour lateral view depicting hgf2 expression in the otic vesicle (arrowhead), pharyngeal arches (arrow), brain ventricles (asterisk), and surrounding the eye (open arrowhead). O: Dorsal view showing 48-hr hgf2 expression in the pharyngeal arches (arrow) and swim bladder (arrowhead). Note weak hgf2 expression in the fin bud (asterisk). P: Later view showing 72-hr hgf2 expression in the pharyngeal arches (arrow) and swim bladder (arrowhead). Q: Seventy-two-hour dorsal view showing swim bladder hgf2 expression (arrowhead) and lack of detectable hgf2 expression near the liver (asterisk). This embryo was cleared in methanol for an extended period so that specific staining could be better visualized. Embryos in E-J and N-Q were treated with PTU to reduce pigment.
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