Cell division of parapineal precursors is largely complete by 18 s. A-D: Dorsal views of 4 d larvae, with BrdU (red) and foxd3:gfp (green) labeling in the epithalamus, following a BrdU pulse at (A, B) 15 s or (C, D) 24 hr stage in (A, C) WT or (B, D) flh mutant embryos. Dashed circles in B, D indicate the pineal cells. Scale bar = 25 μM. E: Line graph of the percentage of WT parapineal (red line) or pineal (blue line) cells that are labeled at 4 d, following a BrdU pulse at the indicated developmental stages. F: Bar graph showing the absolute number of BrdU-positive parapineal cells in WT or flh embryos. G: BrdU-positive pineal cells in WT or flh embryos.
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