FIGURE

Fig. 7

ID

ZDB-FIG-081202-14

Publication

Munson et al., 2008 - Regulation of neurocoel morphogenesis by Pard6gammab

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Fig. 7

Role of the Par3/Par6/aPKC complex in apical membrane formation in the neural tube. All images represent transverse sections between the first and sixth somite stained for ZO-1 (blue), aPKC (red), and filamentous actin (green) at 24 hpf. (A) pard6γb^s441 mutant neural tubes show a reduced amount of apical membranes. (B) pard6γb^s441/prkci double mutants show a more severe reduction in apical membrane formation than pard6γb^s441 mutants. (C) pard6γb^s441 mutants injected with a pard3 morpholino show a more severe reduction in apical membrane formation than pard6γb^s441 mutants. (D) mpp5 mutant neural tubes do not show an obvious severe reduction in apical membrane formation. (E) pard6γb^s441/mpp5 double mutants show a more severe reduction in apical membrane formation than pard6γb^s441 or mpp5 single mutants. In addition, pard6γb^s441/prkci and pard6γb^s441/mpp5 double mutants as well as pard6γb^s441 mutants injected with a pard3 morpholino exhibit a severe epithelial disorganization in the neural tube. Scale bars represent 50 μm.

Expression Data

Antibodies:	Ab1-prkca Ab1-tjp1
Fish:	pals1a^m520/m520 pard6gb^s441/s441 pals1a^m520/m520; pard6gb^s441/s441 pard6gb^s441/s441; prkci^m567/m567
Anatomical Term:	neural tube epithelial cell
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	pals1a^m520/m520 pard6gb^s441/s441 pals1a^m520/m520; pard6gb^s441/s441 pard6gb^s441/s441; prkci^m567/m567
Observed In:	central canal neural tube neural tube formation
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 324(1), Munson, C., Huisken, J., Bit-Avragim, N., Kuo, T., Dong, P.D., Ober, E.A., Verkade, H., Abdelilah-Seyfried, S., and Stainier, D.Y., Regulation of neurocoel morphogenesis by Pard6gammab, 41-54, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.