Expression of the ar gene in the adult zebrafish brain. A: Schematic of brain (modified from Wullimann et al.,[1996]), lateral view, anterior to the left, illustrating position of coronal sections shown in subsequent panels. B,C: low magnification of ventral brains hybridized with antisense (B) or sense probes (C) before sectioning. D-H: Whole brain brightfield images. Panels labeled D′, D″, E′, and so on, are high magnification differential interference contrast (DIC) images of the boxed regions from the corresponding sections. Dorsal to the top. D: Expression in the anterior parvocellular preoptic nucleus (PPa, D′) and in the lateral zone (Dl) of dorsal telencephalon (Tel, D″). E: Cells lining the diencephalic ventricle (DiV, E′), including the periventricular nucleus of posterior tuberculum (TPp) and the ventral zone of periventricular hypothalamus (Hv, E″) express ar. F-H: Expression in the periventricular gray zone of the optic tectum (PGZ, F″) and throughout the hypothalamus (F′,G′,H′). I,J: Brains from Tg(h2afv:GFP)kca66 adults were analyzed for GFP fluorescence (I) or gfp gene expression (J) to confirm that RNA probes penetrated deep regions of the hypothalamus. I, epifluorescence image overlayed onto DIC image. CC, crista cerebellaris; CCe, corpus cerebelli; Hc, caudal zone of the periventricular hypothalamus; Hd, dorsal zone of periventricular hypothalamus; IL, inferior lobe of hypothalamus; LH, lateral hypothalamus; LR, lateral recess of DiV; OB, olfactory bulb; ON, optic nerve; PVO, paraventricular organ; TeO, optic tectum; TH, tuberal hypothalamus. Panels D, F-H are of female brains, panel E are male brains. Scale bars = 200 μm in D-H, D′, D″, E′, and so on, 50 μm in I,J.
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