Elimination of endoderm development in osr1 morphants rescues proximal pronephric phenotype. (A-D) Expression of pax2a in control (A), osr1 morphant (B), sox32 morphant (C) and osr1+sox32 double morphants (D). Loss of osr1 resulted in loss of proximal pax2a pronephric expression (arrowhead, B) when compared with control 18-somite stage embryos (arrowhead, A). sox32 (cas) morphants lacking endoderm showed slightly enhanced expression of pax2a in the proximal nephron (arrowhead, C). The loss of pax2a expression in the proximal pronephros of osr1 morphants was rescued by elimination of endoderm in the osr1+ sox32 double morphants (arrowhead, D). (E-G) Expression of pax2a in uninjected bon heterozygote incross embryos (E) and osr1 morpholino injected bon incross embryos (F,G) at 24 hpf. pax2a expression was lost from the proximal pronephros in 47 out of 64 of the injected embryos (73%) (F). Loss of pax2a expression from the proximal pronephros was rescued in 17 out of 64 (27%) osr1 MO injected, bon heterozygote incross embryos. Arrowheads in E-G indicate expression of pax2a in the proximal pronephros.
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