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ZFIN ID: ZDB-FIG-080917-868
Piotrowski et al., 2000 - The endoderm plays an important role in patterning the segmented pharyngeal region in zebrafish (Danio rerio). Developmental Biology   225(2):339-356 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Terms:
Stage Range: 14-19 somites to Prim-15
PHENOTYPE:
Fish:
Observed In:
Stage Range: Prim-5 to Protruding-mouth

Fig. 8 (A–G) In situ hybridization with dlx-2, a gene which is expressed in neural crest cells, nuclei in the forebrain, and pectoral fins. At 18 somites dlx-2 is expressed in three neural crest streams (A, s1, s2, s3, dorsal view). In 33-hpf wild-type larvae (B, lateral view; D, dorsal view) neural crest cells have populated the arches, which are separated from each other by the endodermal pouches (nonstaining tissue between the arches). Arches p5–p7 have not been divided by the endoderm yet. At this stage the endodermal pouches consist of two rows of cells which have not formed a cavity yet. In vgo (C) only the first endodermal pouch is present. In the absence of posterior endodermal pouches the neural crest cells of the posterior pharyngeal arches fuse (C, E). (F and G) Dorsal views of dlx-2 expression in 28-hpf wild-type and oep mutant embryos, respectively. (H) dlx-2 expression pattern in endodermal-deficient cas mutants. No distinct pharyngeal arches form and the neural crest cells migrate to ectopic locations (arrows). In oep (G) and cas (H), neural crest cells of the anterior arches fuse with each other and expression is reduced in the posterior arches. (I, J) Alcian blue-stained cartilage preparations of 3-dpf wt (I) and cas (J) larvae. In cas larvae the cranial cartilages are severely reduced. The only elements present are the anterior parts of the neurocranium (reduced trabeculae (tr) and ethmoid plate (etp)) and remnants of the parachordals (pc) or possibly two pharyngeal cartilages of unknown identity. Abbreviations: s1–s3, neural crest streams 1–3; p1–p7, pharyngeal arches 1–7.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
dlx2a WT standard conditions 14-19 somites forebrain nucleus ISH
14-19 somites neural crest cell ISH
Prim-5 forebrain nucleus ISH
Prim-5 pharyngeal arch ISH
Prim-15 forebrain nucleus ISH
Prim-15 pectoral fin ISH
Prim-15 pharyngeal arch ISH
sox32ta56/ta56 standard conditions Prim-5 forebrain nucleus ISH
Prim-5 neural crest cell ISH
tbx1tm208/tm208 standard conditions Prim-15 forebrain nucleus ISH
Prim-15 pectoral fin ISH
Prim-15 pharyngeal arch ISH
tbx1tu285/tu285 standard conditions Prim-15 forebrain nucleus ISH
Prim-15 pectoral fin ISH
Prim-15 pharyngeal arch ISH
tdgf1unspecified/unspecified standard conditions Prim-5 forebrain nucleus ISH
Prim-5 neural crest cell ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
sox32ta56/ta56 standard conditions Prim-5 neural crest cell mislocalised, abnormal
Prim-5 pharyngeal arch aplastic, abnormal
Protruding-mouth cartilage morphogenesis disrupted, abnormal
Protruding-mouth cranial cartilage decreased size, abnormal
tbx1tm208/tm208 standard conditions Prim-15 cartilage morphogenesis disrupted, abnormal
Prim-15 pharyngeal arch 3-7 neural crest cell fused with pharyngeal arch 3-7 neural crest cell, abnormal
Prim-15 pharyngeal pouch aplastic, abnormal
tbx1tu285/tu285 standard conditions Prim-15 cartilage morphogenesis disrupted, abnormal
Prim-15 pharyngeal arch 3-7 neural crest cell fused with pharyngeal arch 3-7 neural crest cell, abnormal
Prim-15 pharyngeal pouch aplastic, abnormal
tdgf1unspecified/unspecified standard conditions Prim-5 mandibular neural crest fused with hyoid neural crest, abnormal
Prim-5 neural crest cell migration disrupted, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 225(2), Piotrowski, T. and Nüsslein-Volhard, C., The endoderm plays an important role in patterning the segmented pharyngeal region in zebrafish (Danio rerio), 339-356, Copyright (2000) with permission from Elsevier. Full text @ Dev. Biol.