FIGURE

Fig. 2

ID

ZDB-FIG-080828-18

Publication

Goessling et al., 2008 - APC mutant zebrafish uncover a changing temporal requirement for wnt signaling in liver development

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Fig. 2

β-catenin levels mediate differential liver phenotypes in APC mutant zebrafish. (A–D) IHC for β-catenin at 72 hpf (40x, close-up as inset) revealed an increase in both cytoplasmic (APC^+/-: 39.4 ± 13.4% (% of hepatocytes with cytoplasmic β-catenin ± SD) vs. 9.8 ± 3.8%; n = 5, p = 0.0006) and nuclear staining (20.8 ± 8.2% vs. 4.3 ± 1.8%; n = 5, p = 0.0012) in the hepatocytes of APC^+/- embryos compared to wild-type. While liver-specific IHC could not be performed in the APC^-/-embryos, β-catenin staining was widely positive in the region of the endoderm. (E–I) MO (40 μM) injected in the progeny of an APC^+/- incross at the one-cell stage revealed a shift in liver phenotype distribution. (E) A graphical depiction of the shift in distribution of liver phenotypes with correlated genotypes in β-catenin MO injected embryos compared to controls. Distribution of control MO phenotypes (genotype): 59/234 normal (92% APC^+/+, 8% APC^+/-), 114/234 big (APC^+/-), 61/234 absent (APC^-/-); β-catenin MO phenotypes: 156/211 normal (40% APC^+/+, 60% APC^+/-), 32/211 big (APC^+/-), 13/211 absent and 10/211 rescue (APC^-/-). (F–I) Representative lfabp in situ hybridization phenotypes observed in β-catenin MO injected embryos at 72 hpf.

Expression Data

Gene:	fabp10a
Antibody:	Ab2-ctnnb
Fish:	WT apc^hu745/hu745 WT + MO1-ctnnb1 apc^hu745/+ apc^hu745/+ + MO1-ctnnb1 apc^hu745/hu745 + MO1-ctnnb1
Knockdown Reagent:	MO1-ctnnb1
Anatomical Terms:	endoderm hepatocyte cytoplasm hepatocyte nucleus hepatocyte plasma membrane liver
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	apc^hu745/hu745 apc^hu745/+ apc^hu745/+ + MO1-ctnnb1 apc^hu745/hu745 + MO1-ctnnb1
Knockdown Reagent:	MO1-ctnnb1
Observed In:	apoptotic process liver
Stage Range:	Prim-5 to Protruding-mouth

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 320(1), Goessling, W., North, T.E., Lord, A.M., Ceol, C., Lee, S., Weidinger, G., Bourque, C., Strijbosch, R., Haramis, A.P., Puder, M., Clevers, H., Moon, R.T., and Zon, L.I., APC mutant zebrafish uncover a changing temporal requirement for wnt signaling in liver development, 161-174, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.