FIGURE

Fig. S3 I

ID

ZDB-FIG-080804-31

Publication

Emelyanov et al., 2008 - Mifepristone-inducible LexPR system to drive and control gene expression in transgenic zebrafish

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Fig. S3 I

Uniformity and consistency of expression in double transgenic fish that harbor a driver-reporter Ds(promoter:LexPR-LexOP:EGFP) and an effector Ds(cry:ECFP-LOP:mCherry) constructs. Effector construct contains permanent selection marker crystallin:ECFP which produces cyan expression in the lens (thin arrow) visible through the GFP filter set but not through the mCherry filter set. I. Two different driver-reporter lines with the same promoter crossed to the same effector line. The gfap:LexPR-LexOP:EGFP driver-reporter construct harbors the LexPR transactivator sequence under control of the gfap (glial fibrillary acidic protein) promoter that results in expression of the EGFP reporter in the central nervous system. A (lateral view) and C (dorsal view) show several F₂ fish from the cross between the driver line #4 female and the effector line #1 male. B is a progeny of the independent Driver line #6 crossed with the same Effector line #1. There is strong expression in the forebrain (arrowhead) and in the a few segments of the hindbrain (large arrows).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 320(1), Emelyanov, A., and Parinov, S., Mifepristone-inducible LexPR system to drive and control gene expression in transgenic zebrafish, 113-121, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.