Fig. S3 I

Fig. S3 I Uniformity and consistency of expression in double transgenic fish that harbor a driver-reporter Ds(promoter:LexPR-LexOP:EGFP) and an effector Ds(cry:ECFP-LOP:mCherry) constructs. Effector construct contains permanent selection marker crystallin:ECFP which produces cyan expression in the lens (thin arrow) visible through the GFP filter set but not through the mCherry filter set. I. Two different driver-reporter lines with the same promoter crossed to the same effector line. The gfap:LexPR-LexOP:EGFP driver-reporter construct harbors the LexPR transactivator sequence under control of the gfap (glial fibrillary acidic protein) promoter that results in expression of the EGFP reporter in the central nervous system. A (lateral view) and C (dorsal view) show several F₂ fish from the cross between the driver line #4 female and the effector line #1 male. B is a progeny of the independent Driver line #6 crossed with the same Effector line #1. There is strong expression in the forebrain (arrowhead) and in the a few segments of the hindbrain (large arrows).