FIGURE

Fig. 6

ID

ZDB-FIG-080619-7

Publication

Schenck et al., 2001 - A highly conserved protein family interacting with the fragile X mental retardation protein (FMRP) and displaying selective interactions with FMRP-related proteins FXR1P and FXR2P

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Fig. 6

The FMRP sequence encoded by FMR1 exon 7 is implicated in the interaction with CYFIP1. Yeast two-hybrid assay between CYFIP1 and chimeric FMRP/FXR1P and FMRP/FXR2P proteins. In hybrid constructs, 1.1-2.2 increasing parts of FMRP exon 7 were replaced by FXR1P/2P, in hybrids 3.1 and 3.2 the entire exon 7. In construct 4.1, exon 4-7/8 of FMR1 is substituted by the FXR1 sequence. To the right of each construct, the strength of interaction with CYFIP1 in relation to interaction FMRP-CYFIP1 is indicated. For constructs 1.1, 1.2, 3.1, and 3.2, also an X-gal filter lift assay is shown. A more detailed representation of the hybrid constructs, the FMRP exon 7 region, and the corresponding FXR1P/2P sequences is published as supplemental data on the PNAS web site, www.pnas.org.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA