FIGURE

Fig. 3

ID

ZDB-FIG-080619-4

Publication

Schenck et al., 2001 - A highly conserved protein family interacting with the fragile X mental retardation protein (FMRP) and displaying selective interactions with FMRP-related proteins FXR1P and FXR2P

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Fig. 3

Coimmunolocalization studies in transiently transfected Cos cells. Row 1: cells transfected with CYFIP1 and FMRP iso7. FMRP was revealed by mAb 1C3, CYFIP1 by pAb no. 1465. Row 2: cells cotransfected with GFP-CYFIP1 and FMRP iso7, revealed by mAb 1C3. Row 3: cells cotransfected with GFP-tagged CYFIP1 and a myc-tagged CYFIP2 construct, revealed by mAb α-myc 9E10. Row 4: Cos transfected with myc-CYFIP2 (observed by 9E10) and compared with localization of endogenous ribosomes, revealed by a pAb raised against L7a, a protein of the ribosomal large subunit. Anti-L7a also stains nucleoli, the loci of ribosome assembly. Column 3: Hoechst staining. Column 4: merging images (yellow) from series 1 and 2, respectively, indicate colocalization.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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