FIGURE

Fig. 7

ID

ZDB-FIG-080613-7

Publication

Delporte et al., 2008 - Expression of zebrafish pax6b in pancreas is regulated by two enhancers containing highly conserved cis-elements bound by PDX1, PBX and PREP factors

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Fig. 7

PBX-PREP dimers bind to the P0-pax6b PC1 element and PDX1 binds to PC3b as a monomer. (A) EMSAs were performed on PC1 probe of pax6b (PC1b) using nuclear extract of pancreatic TU6 cells. (B) EMSAs were performed on PC3 probe of pax6b (PC3b) using nuclear extract of pancreatic TU6 cells. 10 ng of unlabeled oligonucleotides PC3b, UE-A and mutated UE-A were added as competitor. Abbreviations: PBX1a and PREP1, recombinant PBX1a and PREP1 proteins produced in vitro in a reticulocyte extract; PDX1 Ab, PDX1 antiserum; PBXL. Ab, antiserum recognizing specifically the long PBX isoforms (PBX1a, PBX2, and PBX3a) (Santa Cruz Biotechnology); PREP1 Ab, PREP1 antibody PBX1a and PREP1; P.I. serum, pre-immune serum.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ BMC Dev. Biol.