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Fig. 1

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ZDB-FIG-080513-24

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Hjorth et al., 2001 - Are pioneer axons guided by regulatory gene expression domains in the zebrafish forebrain? High-resolution analysis of the patterning of the zebrafish brain during axon tract formation

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Fig. 1

Gene expression patterns obtained by fluorescence in situ hybridisation utilising the confocal laser scanning microscope correspond to expression patterns obtained using bright-field microscopy. Whole-mount dissected zebrafish brains labelled for gene expression patterns (red) were first photographed using bright-field microscopy (A, C, E) and then visualised using the confocal laser scanning microscope by collecting Z-series images (B, D, F). (A, B) pax6 expression at 20 hpf. (C, D) shh expression at 16 hpf. (E, F) otx2 expression at 18 hpf. Borders of gene expression (arrows), regions of low expression (asterisks), and regions of high expression (arrowheads) corresponded in both bright-field and fluorescent images. Bar, 50 μm.

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Antibody Labeling

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Reprinted from Developmental Biology, 229(2), Hjorth, J.T. and Key, B., Are pioneer axons guided by regulatory gene expression domains in the zebrafish forebrain? High-resolution analysis of the patterning of the zebrafish brain during axon tract formation, 271-286, Copyright (2001) with permission from Elsevier. Full text @ Dev. Biol.