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Fig. 1

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ZDB-FIG-080429-18

Publication

Wu et al., 2002 - Role of PTPase(s) in regulating Fyn kinase at fertilization of the zebrafish egg

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Fig. 1

PTPase inhibitors block Fyn activation at fertilization. Groups of 500 eggs were incubated with Hanks–BSA containing 0.1% DMSO or Hanks–BSA containing 0.1% DMSO and 10 μM peroxyvanadate (POV) for 30 min at 28°C. One group was then fertilized (F) and allowed to develop for 2 min, at which time all samples were homogenized in immunoprecipitation buffer. The detergent extracts were then incubated with anti-Fyn antibody or normal rabbit IgG (Control) and immunoprecipitates were incubated in kinase buffer containing [γ-³²P]ATP and the reaction products resolved by SDS–PAGE. Autophosphorylation was detected by autoradiography over 7 days of exposure, and the position of the 57-kDa Fyn protein is indicated by the arrow.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

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Reprinted from Developmental Biology, 247(2), Wu, W. and Kinsey, W.H., Role of PTPase(s) in regulating Fyn kinase at fertilization of the zebrafish egg, 286-94, Copyright (2002) with permission from Elsevier. Full text @ Dev. Biol.