ZFIN Image: Wu et al., 2002, Fig. 1

Image

Figure Caption

Fig. 1 PTPase inhibitors block Fyn activation at fertilization. Groups of 500 eggs were incubated with Hanks–BSA containing 0.1% DMSO or Hanks–BSA containing 0.1% DMSO and 10 μM peroxyvanadate (POV) for 30 min at 28°C. One group was then fertilized (F) and allowed to develop for 2 min, at which time all samples were homogenized in immunoprecipitation buffer. The detergent extracts were then incubated with anti-Fyn antibody or normal rabbit IgG (Control) and immunoprecipitates were incubated in kinase buffer containing [γ-³²P]ATP and the reaction products resolved by SDS–PAGE. Autophosphorylation was detected by autoradiography over 7 days of exposure, and the position of the 57-kDa Fyn protein is indicated by the arrow.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 247(2), Wu, W. and Kinsey, W.H., Role of PTPase(s) in regulating Fyn kinase at fertilization of the zebrafish egg, 286-94, Copyright (2002) with permission from Elsevier. Full text @ Dev. Biol.